Abstract The Nilaparvata muiri (Hemiptera: Delphacidae) is a sibling species of a destructive rice insect pest, the brown planthopper (BPH), Nilaparvata lugens.Here, we generated a high-quality chromosome-level genome assembly of N.muiri using a combination of the PacBio HiFi sequencing, Illumina short-read sequencing and Hi-C scaffolding technologies.The genome assembly (524.
9 Mb) is anchored to 15 Bullet Valve pseudochromosomes, with a scaffold N50 of 43.3 Mb CHAMOMILE FLOWERS and 99.1% BUSCO completeness.It contains 188.
1 Mb repeat sequences and 13204 protein-coding genes.As a closely related species within the same genus as the significant pest, N.lugens, the chromosome-level genome assembly of N.muiri will provide important support for the better analysis of pathogenicity mechanisms of N.
lugens based on comparative genomics.